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TransGen biotech co transscript reverse transcriptase
Transscript Reverse Transcriptase, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transscript+reverse+transcriptase/transscript+reverse+transcriptase/pmc11294541-142-34-37
Average 90 stars, based on 1 article reviews
transscript reverse transcriptase - by Bioz Stars, 2026-11
90/100 stars

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Transfection:

Article Title: Pathogenic Analysis of Two SLC22A5 Variants That Alter RNA Splicing in Patients with Primary Carnitine Deficiency.
Article Snippet: Both WT and mutant minigene plasmids were transformed into Escherichia coli DH5α-competent cells (DL1080, WEIDI, Shanghai, China); the constructed WT and mutant sequences were confirmed by Sanger sequencing. https://doi.org/10.3390/ijns12010017 The constructs were then transfected into human embryonic kidney 293 cells and HeLa cells using Hieff Trans® Liposomal 2000 Transfection Reagent (40802ES03, YEASEN, Shanghai, China) following the manufacturer’s protocol. .. After 48 h of transfection, the cells were harvested, and total RNA was extracted and reverse transcribed into complementary DNAs (cDNAs) using TransScript Reverse Transcriptase (RR092B, Takara, Kusatsu, Japan). ..

Article Title: Pathogenic Analysis of Two SLC22A5 Variants That Alter RNA Splicing in Patients with Primary Carnitine Deficiency
Article Snippet: The constructs were then transfected into human embryonic kidney 293 cells and HeLa cells using Hieff Trans ® Liposomal 2000 Transfection Reagent (40802ES03, YEASEN, Shanghai, China) following the manufacturer’s protocol. .. After 48 h of transfection, the cells were harvested, and total RNA was extracted and reverse transcribed into complementary DNAs (cDNAs) using TransScript Reverse Transcriptase (RR092B, Takara, Kusatsu, Japan). ..

Reverse Transcription:

Article Title: Pathogenic Analysis of Two SLC22A5 Variants That Alter RNA Splicing in Patients with Primary Carnitine Deficiency.
Article Snippet: Both WT and mutant minigene plasmids were transformed into Escherichia coli DH5α-competent cells (DL1080, WEIDI, Shanghai, China); the constructed WT and mutant sequences were confirmed by Sanger sequencing. https://doi.org/10.3390/ijns12010017 The constructs were then transfected into human embryonic kidney 293 cells and HeLa cells using Hieff Trans® Liposomal 2000 Transfection Reagent (40802ES03, YEASEN, Shanghai, China) following the manufacturer’s protocol. .. After 48 h of transfection, the cells were harvested, and total RNA was extracted and reverse transcribed into complementary DNAs (cDNAs) using TransScript Reverse Transcriptase (RR092B, Takara, Kusatsu, Japan). ..

Article Title: Genome-wide analysis of the common bean (Phaseolus vulgaris) laccase gene family and its functions in response to abiotic stress.
Article Snippet: RNA quality was assessed via gel electrophoresis and an Agilent 2100 bioanalyzer. .. First-strand cDNA synthesis was conducted using TransScript reverse transcriptase (TaKaRa, Dalian, China) following the manufacturer’s guidelines. ..

Article Title: The Citrus Laccase Gene CsLAC18 Contributes to Cold Tolerance.
Article Snippet: .. The first strand cDNA was performed by TransScript Reverse transcriptase (TaKaRa, Dalian, China), following the manufacturer’s instructions. ..

Article Title: Genome-wide analysis of the common bean ( Phaseolus vulgaris ) laccase gene family and its functions in response to abiotic stress
Article Snippet: RNA quality was assessed via gel electrophoresis and an Agilent 2100 bioanalyzer. .. First-strand cDNA synthesis was conducted using TransScript reverse transcriptase (TaKaRa, Dalian, China) following the manufacturer’s guidelines. ..

Article Title: Upregulation of neuronal ER-phagy improves organismal fitness and alleviates APP toxicity.
Article Snippet: RNA isolation, semiquantitative RT-PCR, and quantitative real-time PCR Total RNAwas extracted from aminimum of 10 third-instar larvae and 20 fly heads, isolated using TRIzol (Cat# 15596026, Invitrogen). .. Then, 1 mg of total RNA was reverse transcribed into cDNA using the TransScript Reverse Transcriptase (HiScript III RT SuperMix, Cat# R323-01, Vazyme). ..

Article Title: Sequence-specific nanoparticle barcode strategy for multiplex human enterovirus typing
Article Snippet: .. PCR was performed in a 25 μL reaction system, which contained 50 mM Tris-HCl (pH 8.5), 2 mM MgSO4, 70 mM CH3COOK, 200 μM dNTPs, 2 U Taq HS DNA polymerase (TaKaRa), 4 U TransScript Reverse Transcriptase (TransGen Biotech), 0.4 μM of universal primers, and 5 μL of template. .. Thermal cycling consisted of the reverse transcription step at 50 °C for 10 min, an enzyme activation step at 95 °C for 5 min, followed by thirty cycles of 95 °C for 15 s, and 55 °C for 45 s. PCR was performed on a T100 thermal cycler (Bio-Rad).

Article Title: Comparative Transcriptome and Metabolome Profiling Revealed Molecular Cascade Events During the Enzymatic Browning of Potato Tubers After Cutting.
Article Snippet: .. First-strand cDNA was synthesized from 2 μg total RNA using Tiangen TransScript reverse transcriptase. qRT-PCR was conducted using Premix Ex TaqTM (Takara, Japan) on an Applied Biosystems StepOnePlus thermocycler (Applied Biosystems, Beverly, MA, USA). ..

Article Title: Pathogenic Analysis of Two SLC22A5 Variants That Alter RNA Splicing in Patients with Primary Carnitine Deficiency
Article Snippet: The constructs were then transfected into human embryonic kidney 293 cells and HeLa cells using Hieff Trans ® Liposomal 2000 Transfection Reagent (40802ES03, YEASEN, Shanghai, China) following the manufacturer’s protocol. .. After 48 h of transfection, the cells were harvested, and total RNA was extracted and reverse transcribed into complementary DNAs (cDNAs) using TransScript Reverse Transcriptase (RR092B, Takara, Kusatsu, Japan). ..

cDNA Synthesis:

Article Title: Genome-wide analysis of the common bean (Phaseolus vulgaris) laccase gene family and its functions in response to abiotic stress.
Article Snippet: RNA quality was assessed via gel electrophoresis and an Agilent 2100 bioanalyzer. .. First-strand cDNA synthesis was conducted using TransScript reverse transcriptase (TaKaRa, Dalian, China) following the manufacturer’s guidelines. ..

Article Title: Genome-wide analysis of the common bean ( Phaseolus vulgaris ) laccase gene family and its functions in response to abiotic stress
Article Snippet: RNA quality was assessed via gel electrophoresis and an Agilent 2100 bioanalyzer. .. First-strand cDNA synthesis was conducted using TransScript reverse transcriptase (TaKaRa, Dalian, China) following the manufacturer’s guidelines. ..

Polymerase Chain Reaction:

Article Title: Sequence-specific nanoparticle barcode strategy for multiplex human enterovirus typing
Article Snippet: .. PCR was performed in a 25 μL reaction system, which contained 50 mM Tris-HCl (pH 8.5), 2 mM MgSO4, 70 mM CH3COOK, 200 μM dNTPs, 2 U Taq HS DNA polymerase (TaKaRa), 4 U TransScript Reverse Transcriptase (TransGen Biotech), 0.4 μM of universal primers, and 5 μL of template. .. Thermal cycling consisted of the reverse transcription step at 50 °C for 10 min, an enzyme activation step at 95 °C for 5 min, followed by thirty cycles of 95 °C for 15 s, and 55 °C for 45 s. PCR was performed on a T100 thermal cycler (Bio-Rad).

Synthesized:

Article Title: Comparative Transcriptome and Metabolome Profiling Revealed Molecular Cascade Events During the Enzymatic Browning of Potato Tubers After Cutting.
Article Snippet: .. First-strand cDNA was synthesized from 2 μg total RNA using Tiangen TransScript reverse transcriptase. qRT-PCR was conducted using Premix Ex TaqTM (Takara, Japan) on an Applied Biosystems StepOnePlus thermocycler (Applied Biosystems, Beverly, MA, USA). ..

Quantitative RT-PCR:

Article Title: Comparative Transcriptome and Metabolome Profiling Revealed Molecular Cascade Events During the Enzymatic Browning of Potato Tubers After Cutting.
Article Snippet: .. First-strand cDNA was synthesized from 2 μg total RNA using Tiangen TransScript reverse transcriptase. qRT-PCR was conducted using Premix Ex TaqTM (Takara, Japan) on an Applied Biosystems StepOnePlus thermocycler (Applied Biosystems, Beverly, MA, USA). ..



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